Mitochondrial metabolism promotes adaptation to proteotoxic stress

This study was focused on understanding the mechanism cells use to adapt to proteotoxic stress. Part of the work used a panel of 549 PRISM cell lines grown in either glucose or galactose (to increase dependency on mitochondrial metabolism) in the presence or absence of bortezomib. Increased mitochondrial metabolism promoted proteasome inhibitor resistance.

The landscape of cancer cell line metabolism

This work sought to create a  comprehensive resource of the metabolic diversity of cancer. Part of the study involved pooling 544 adherent PRISM cell lines which were then grown under media conditions with varied amino acid concentration to understand the effect on cell viability.

A metastasis map of human cancer cell lines

This work details the creation of a first-generation metastasis map to reveal organ-specific patterns of metastasis of 500 PRISM cell lines. This was done by injecting pools of 5 cell lines into mouse xenograft models. Results were used to develop the MetMap resource (

Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery

This paper introduces a novel ‘nanoPRISM’ method that leverages the PRISM cell set to understand the structure-function relationship of nanoparticles. For this study, the PR500 was treated with a library of modular fluorescent nanoparticles to understand regulators of nanoparticle delivery. In particular, SLC46A3 (a lysosomal transporter) was identified as a negative regulator of lipid-based nanoparticle […]